PIK3C2A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PIK3C2A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Rezensionen (1)

Kat-Nr. : 22028-1-AP

Synonyme

CPK, PI 3 Kinase Class 2A, PI 3-kinase C2alpha, PI3 K C2(ALPHA), PI3 K C2A, PI3K C2 alpha, PIK3C2A

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  WB analysis of HeLa using 22028-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22028-1-AP

  WB result of PIK3C2A antibody (22028-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIK3C2A transfected HeLa cells.

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  WB analysis of PC-3 using 22028-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22028-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 22028-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 22028-1-AP

  IP result of anti-PIK3C2A (IP:22028-1-AP, 4ug; Detection:22028-1-AP 1:15000) with PC-3 cells lysate 1480 ug.

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  IHC staining of mouse ovary using 22028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 22028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 22028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 22028-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PIK3C2A antibody (22028-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Plazenta-Gewebe, PC-3-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: Maus-Eierstockgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Produktinformation

22028-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PIK3C2A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIK3C2A fusion protein Ag16894
• Vollständiger Name: phosphoinositide-3-kinase, class 2, alpha polypeptide
• Berechnetes Molekulargewicht: 1686 aa, 191 kDa
• Beobachtetes Molekulargewicht: 191 kDa
• GenBank-Zugangsnummer: BC113658
• Gene symbol: PIK3C2A
• Gene ID (NCBI): 5286
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Science; PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.; Authors - Federico Gulluni ; KD Validated
human	WB, IF	Cancer Cell; Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function.; Authors - Federico Gulluni
rat	WB,IF	Cell Res; Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation.; Authors - Shu-Xin Zhang
human	WB,IF	J Cell Sci; Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization; Authors - Ellis L Ryan ; KD Validated
human	IF, WB	J Cell Sci; Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization.; Authors - Ellis L Ryan
human	WB	Front Cell Infect Microbiol; The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection.; Authors - Teng Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

HUAYI (Verified Customer) (01-17-2020)

This antibody provide brilliant Western Blot results, and as well, the specificity for Immunofloruorence is high. But we are glad if more discounts would be received.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cell lines

PIK3C2A Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in cancer cell lines (Cat no:22028-1-AP)