PI3 Kinase p110 Alpha Polyklonaler Antikörper

PI3 Kinase p110 Alpha Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 21890-1-AP

Synonyme

p110 alpha, PI3 Kinase p110 Alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110(alpha), PI3-kinase p110 subunit alpha, PIK3CA, PtdIns 3 kinase subunit alpha

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Galerie der Validierungsdaten

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  WB analysis of human brain using 21890-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21890-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 21890-1-AP

  IP result of anti-PI3K p110 (alpha) (IP:21890-1-AP, 4ug; Detection:21890-1-AP 1:300) with mouse heart tissue lysate 3600 ug.

• 

  IHC staining of human lung cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21890-1-AP (PI3K p110(alpha) antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21890-1-AP (PI3K p110(alpha) antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21890-1-AP (PI3K p110(alpha) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21890-1-AP (PI3K p110(alpha) antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 21890-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 21890-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug PI3K p110(alpha) antibody (21890-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Jurkat-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below
• IHC: See 1 publications below

Produktinformation

21890-1-AP bindet in WB, IP, IHC, IF, FC, ELISA PI3 Kinase p110 Alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PI3 Kinase p110 Alpha fusion protein Ag16586
• Vollständiger Name: phosphoinositide-3-kinase, catalytic, alpha polypeptide
• Berechnetes Molekulargewicht: 1068 aa, 124 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC113601
• Gene symbol: PIK3CA
• Gene ID (NCBI): 5290
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

Publikationen

Species	Application	Title
rat	WB	Food Funct; l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways; Authors - Ling Lin
human	WB	J Agric Food Chem; β-Sitosterol-D-glucoside from sweet potato exerts an anti-breast cancer activity by upregulating microRNA-10a and PI3K/Akt signaling pathway.; Authors - Heshan Xu
human	WB	J Hematol Oncol; Long non-coding RNA-SNHG7 acts as a target of miR-34a to increase GALNT7 level and regulate PI3K/Akt/mTOR pathway in colorectal cancer progression.; Authors - Yang Li
mouse	WB,IHC	Sci Rep; NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.; Authors - Shu Yang
human	WB	J Exp Clin Cancer Res; LINC01296/miR-26a/GALNT3 axis contributes to colorectal cancer progression by regulating O-glycosylated MUC1 via PI3K/AKT pathway.; Authors - Bing Liu
mouse	WB	Sci Rep; Hypoxia-induced ANGPTL4 sustains tumour growth and anoikis resistance through different mechanisms in scirrhous gastric cancer cell lines.; Authors - Koichi Baba

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