PI3 Kinase p110 Beta Polyklonaler Antikörper

PI3 Kinase p110 Beta Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 21739-1-AP

Synonyme

p110 BETA, PI3 Kinase p110 Beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3K p110(beta), PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta

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Galerie der Validierungsdaten

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  IHC staining of human liver cancer using 21739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21739-1-AP (PI3K p110(beta) antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 21739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21739-1-AP (PI3K p110(beta) antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 21739-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21739-1-AP (PI3K p110(beta) antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 21739-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21739-1-AP (PI3K p110(beta) antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 21739-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21739-1-AP (PI3K p110(beta) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

21739-1-AP bindet in WB, IHC, IF, ELISA PI3 Kinase p110 Beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PI3 Kinase p110 Beta fusion protein Ag16456
• Vollständiger Name: phosphoinositide-3-kinase, catalytic, beta polypeptide
• Berechnetes Molekulargewicht: 1070 aa, 123 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC114432
• Gene symbol: PIK3CB
• Gene ID (NCBI): 5291
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002).

Publikationen

Species	Application	Title
human,mouse	WB	Cell Mol Gastroenterol Hepatol; Mannan-binding lectin via interaction with cell surface calreticulin promotes senescence of activated hepatic stellate cells to limit liver fibrosis progression.; Authors - Jialiang Luo
human	WB	Biochem Pharmacol; TANGO1 interacts with NRTN to promote hepatocellular carcinoma progression by regulating the PI3K/AKT/mTOR signaling pathway; Authors - Jing Man
human	WB	J Ethnopharmacol; Root extract of Hemsleya amabilis Diels suppresses renal cell carcinoma cell growth through inducing apoptosis and G2/M phase arrest via PI3K/AKT signaling pathway; Authors - Kai Li
human	WB	Cells; Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.; Authors - Damian Kołat
human	WB	J Zhejiang Univ Sci B; Silencing of DsbA-L gene impairs the PPARγ agonist function of improving insulin resistance in a high-glucose cell model.; Authors - Xuan Zhou
		Mol Med Rep; EGFR‑associated pathways involved in traditional Chinese medicine (TCM)‑1‑induced cell growth inhibition, autophagy and apoptosis in prostate cancer.; Authors - Zhaomeng Wu