PI3 Kinase p110 Beta Monoklonaler Antikörper

PI3 Kinase p110 Beta Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(29)

CloneNo.

1B2A1

Kat-Nr. : 67121-1-Ig

Synonyme

PI3K p110(beta), PIK3CB, PI3K, PI3 kinase subunit beta, p110 BETA

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  WB analysis using 67121-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67121-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67121-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67121-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67121-1-Ig (PIK3CB antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67121-1-Ig (PIK3CB antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67121-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PI3 Kinase p110 Beta antibody (67121-1-Ig, Clone: 1B2A1 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen, PC-12-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Zervixkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 27 publications below
• IHC: See 5 publications below

Produktinformation

67121-1-Ig bindet in WB, IHC, IF/ICC, ELISA PI3 Kinase p110 Beta und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PI3 Kinase p110 Beta fusion protein Ag17505
• Vollständiger Name: phosphoinositide-3-kinase, catalytic, beta polypeptide
• Berechnetes Molekulargewicht: 1070 aa, 123 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC114432
• Gene symbol: PI3 Kinase p110 Beta
• Gene ID (NCBI): 5291
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002).

Publikationen

Species	Application	Title
mouse	WB	Nat Metab; Maternal exercise prevents metabolic disorders in offspring mice through SERPINA3C; Authors - Yang Li
human	WB	J Med Chem; Discovery of Novel Phosphoinositide-3-Kinase α Inhibitors with High Selectivity, Excellent Bioavailability, and Long-Acting Efficacy for Gastric Cancer.; Authors - Yi Hou
human	WB	J Ethnopharmacol; Antitumor mechanism of kangliu pill on gliomas in mice through PI3K-Akt signaling pathway; Authors - Fei Chen
human	WB,IHC	Front Oncol; Relationship Between PTEN and Angiogenesis of Esophageal Squamous Cell Carcinoma and the Underlying Mechanism.; Authors - Chenbo Yang
human	WB	Bioorg Chem; Design, synthesis and biological evaluation of anilide (dicarboxylic acid) shikonin esters as antitumor agents through targeting PI3K/Akt/mTOR signaling pathway.; Authors - Yingying Ma
mouse	WB	Chin Med; Integrated metabolomics, network pharmacology and biological verification to reveal the mechanisms of Nauclea officinalis treatment of LPS-induced acute lung injury; Authors - Han Xu