PIK3IP1 Polyklonaler Antikörper

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• KD/KO Validated

PIK3IP1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 16826-1-AP

Synonyme

HGFL, hHGFL(S), PIK3IP1

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  WB analysis of HeLa using 16826-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16826-1-AP (PIK3IP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16826-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16826-1-AP (PIK3IP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16826-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16826-1-AP (PIK3IP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16826-1-AP

  IP result of anti-PIK3IP1 (IP:16826-1-AP, 4ug; Detection:16826-1-AP 1:500) with HeLa cells lysate 3200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Mauslebergewebe
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 15 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

16826-1-AP bindet in WB, IF, IP, CoIP, ELISA PIK3IP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIK3IP1 fusion protein Ag10427
• Vollständiger Name: phosphoinositide-3-kinase interacting protein 1
• Berechnetes Molekulargewicht: 263 aa, 28 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC011049
• Gene symbol: PIK3IP1
• Gene ID (NCBI): 113791
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIK3IP1(Phosphoinositide-3-kinase-interacting protein 1) is also named as HGFL. The class IA phosphoinositol-3-kinases (PI3Ks) regulate important cellular processes such as proliferation, growth, survival, motility and metabolism. PIK3IP1 is a transmembrane protein that possesses a region in its intracellular domain that shares homology with the p85 regulatory subunit of PI3K. It has 5 isoforms produced by alternative splicing with the MW of 11, 18, 19, 25,28 kDa. PIK3IP1 undergoes N- and O-linked amino acid glycosylation. The major glycosylated form of PIK3IP1 migrates at about 43 kDa in western blot analysis, while the unglycosylated form migrates at 37 kDa. It is also detected a 65 kDa variant in HepG2 cell lysate that PIK3IP1 is indeed membrane bound and that these variants arise from alternative splicing and/or post-translational processing events such as enzymatic proteolysis and/or glycosylation. (PMID:18632611).

Publikationen

Species	Application	Title
human	WB	Nat Genet; Inactivating CUX1 mutations promote tumorigenesis.; Authors - Chi C Wong
		Cell; Distinct Regulation of Th17 and Th1 Cell Differentiation by Glutaminase-Dependent Metabolism.; Authors - Marc O Johnson
mouse	WB	Nat Commun; Dual blockade of the lipid kinase PIP4Ks and mitotic pathways leads to cancer-selective lethality.; Authors - Mayumi Kitagawa ; KD Validated
human	WB	Oncogene; PPM1D activity promotes cellular transformation by preventing senescence and cell death; Authors - Miroslav Stoyanov
mouse	WB	J Immunol; Cutting Edge: Foxp1 Controls Naive CD8+ T Cell Quiescence by Simultaneously Repressing Key Pathways in Cellular Metabolism and Cell Cycle Progression.; Authors - Hairong Wei
mouse	WB	Sci Rep; Downregulation of PIK3IP1 in retinal microglia promotes retinal pathological neovascularization via PI3K-AKT pathway activation; Authors - Lushu Chen