PI3 Kinase p85 Alpha Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PI3 Kinase p85 Alpha Monoklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(257)

CloneNo.

4G3C11

Kat-Nr. : 60225-1-Ig

Synonyme

P85a; PIK3R1, PI3K p85 alpha, PI3K p85 α, PIK3R1, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha

---

• 

  WB analysis using 60225-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60225-1-Ig

  WB result of PI3 Kinase p85 Alpha antibody (60225-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI3 Kinase p85 Alpha transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 60225-1-Ig

  WB result of PI3 Kinase p85 Alpha antibody (60225-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI3 Kinase p85 Alpha transfected HEK-293 cells.

• 

  WB analysis using 60225-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60225-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60225-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 60225-1-Ig

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60225-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60225-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 60225-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60225-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 60225-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 60225-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60225-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 60225-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 60225-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 60225-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of RAW 264.7 using 60225-1-Ig

  IP result of anti-PI3 Kinase p85 Alpha (IP:60225-1-Ig, 5ug; Detection:60225-1-Ig 1:500) with RAW 264.7 cells lysate 1480ug.

• 

  IHC staining of mouse lung using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate cancer using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, C2C12-Zellen, fetales humanes Hirngewebe, HT-29-Zellen, Jurkat-Zellen, Maushirngewebe, Neuro-2a-Zellen, PC-12-Zellen, Hausschwein-Hirngewebe, Hausschwein-Skelettmuskelgewebe, Kaninchenhirngewebe, Rattenhirngewebe, Ratten-Skelettmuskelgewebe, U-251-Zellen
• Erfolgreiche IP: RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Prostatakarzinomgewebe, humanes Skelettmuskelgewebe, Maushirngewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 249 publications below
• IHC: See 12 publications below
• IF: See 3 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

60225-1-Ig bindet in WB, IHC, IF, IP, CoIP, ELISA PI3 Kinase p85 Alpha und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, Hausschwein, Hund, Maus, Ratte, Rind
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PI3 Kinase p85 Alpha fusion protein Ag2344
• Vollständiger Name: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC030815
• Gene symbol: PI3 Kinase p85 Alpha
• Gene ID (NCBI): 5295
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphatidylinositol 3-kinase (PI3K) plays an important role in the metabolic actions of INS and is required for adipogenesis. The PI3K pathway has also been identified as an important player in cancer development and progression. The Class IA PI3K heterodimer, which is composed of a P110 catalytic subunit and a P85 regulatory subunit, is activated upon association of the P85 subunit with upstream adaptor proteins or receptor tyrosine kinases. Mutations in PIK3R1 are implicated in cases of breast cancer and associated to SHORT syndrome.

Publikationen

Species	Application	Title
human	WB	Biomater Res; High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration; Authors - Di Sun
human	WB	Autophagy; The P2RY12 receptor promotes VSMC-derived foam cell formation by inhibiting autophagy in advanced atherosclerosis.; Authors - Shulan Pi
human	WB	Adv Healthc Mater; Bioelectrically Reprogramming Hydrogels Rejuvenate Vascularized Bone Regeneration in Senescence; Authors - Tianli Wu
mouse	WB	Anesthesiology; Nerve Growth Factor/Tyrosine Kinase A Receptor Pathway Enhances Analgesia in an Experimental Mouse Model of Bone Cancer Pain by Increasing Membrane Levels of δ-Opioid Receptors; Authors - Bu-Fan Kan
mouse	WB	Cell Commun Signal; BMAL1 ameliorates type 2 diabetes-induced cognitive impairment via AREG upregulation and PI3K/Akt/GSK-3β pathway activation; Authors - Jialu Xu
mouse	WB	Int J Biol Macromol; Astrocyte-conditional knockout of MOB2 inhibits the phenotypic conversion of reactive astrocytes from A1 to A2 following spinal cord injury in mice; Authors - Xin Tao