PI3 Kinase p85 Beta Rekombinanter Antikörper

PI3 Kinase p85 Beta Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240531B5

Kat-Nr. : 83606-5-PBS

Synonyme

PI3K p85 β, PI3K p85(beta), PI3K p85β, PIK3R2, p85β

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  WB analysis using 83606-5-RR (same clone as 83606-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83606-5-RR (PIK3R2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83606-5-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 83606-5-RR (same clone as 83606-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PIK3R2 antibody (83606-5-RR, Clone: 240531B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83606-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83606-5-RR (same clone as 83606-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PIK3R2 antibody (83606-5-RR, Clone: 240531B5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83606-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83606-5-PBS bindet in WB, IF/ICC, ELISA PI3 Kinase p85 Beta und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PI3 Kinase p85 Beta fusion protein Ag14367
• Vollständiger Name: phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• Berechnetes Molekulargewicht: 728 aa, 82 kDa
• Beobachtetes Molekulargewicht: 82 kDa
• GenBank-Zugangsnummer: BC014170
• Gene symbol: PI3 Kinase p85 Beta
• Gene ID (NCBI): 5296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIK3R2 is also named as PI3 Kinase p85 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).