PIN1 Polyklonaler Antikörper

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• KD/KO Validated

PIN1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10495-1-PBS

Synonyme

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• 

  WB analysis of HEK-293 using 10495-1-AP (same clone as 10495-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10495-1-AP (same clone as 10495-1-PBS)

  WB result of PIN1 antibody (10495-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIN1 transfected HeLa cells. This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10495-1-AP (same clone as 10495-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10495-1-AP (same clone as 10495-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10495-1-AP (same clone as 10495-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10495-1-AP (same clone as 10495-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 10495-1-AP (same clone as 10495-1-PBS)

  IP result of anti-PIN1 (IP:10495-1-AP, 4ug; Detection:10495-1-AP 1:500) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 10495-1-AP (same clone as 10495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 10495-1-AP (same clone as 10495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 10495-1-AP (same clone as 10495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 10495-1-AP (same clone as 10495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 10495-1-AP (same clone as 10495-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 10495-1-AP (PIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 10495-1-AP (same clone as 10495-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 10495-1-AP (same clone as 10495-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 10495-1-AP (same clone as 10495-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10495-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10495-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PIN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIN1 fusion protein Ag0767
• Vollständiger Name: peptidylprolyl cis/trans isomerase, NIMA-interacting 1
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC002899
• Gene symbol: PIN1
• Gene ID (NCBI): 5300
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

• PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).

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