PIN4 Polyklonaler Antikörper

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• KD/KO Validated

PIN4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 15789-1-AP

Synonyme

Peptidyl-prolyl cis/trans isomerase EPVH, Parvulin-17, Parvulin-14, hPar14, EPVH

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  WB analysis of mouse liver using 15789-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15789-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15789-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15789-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15789-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15789-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15789-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15789-1-AP (PIN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15789-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15789-1-AP (PIN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15789-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15789-1-AP (PIN4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 15789-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15789-1-AP (PIN4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15789-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15789-1-AP (PIN4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15789-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15789-1-AP (PIN4 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 15789-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PIN4 antibody (15789-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HeLa-Zellen, Maushirngewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

15789-1-AP bindet in WB, IHC, IF/ICC, ELISA PIN4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIN4 fusion protein Ag8489
• Vollständiger Name: protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin)
• Berechnetes Molekulargewicht: 131 aa, 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC005234
• Gene symbol: PIN4
• Gene ID (NCBI): 5303
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIN4, also known as EPVH, hPar14, is a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. PIN4 is the first eukaryotic member of the parvulin family of PPIases and contains a PPIase domain preceded by a 40-amino acid glycine- and lysine-rich N-terminal sequence.

Publikationen

Species	Application	Title
human	WB	Cancer Med; Par14 interacts with the androgen receptor, augmenting both its transcriptional activity and prostate cancer proliferation; Authors - Miki Naito ; KD Validated