PINK1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PINK1 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23274-1-PBS

Synonyme

BRPK, EC:2.7.11.1, PARK6, PTEN induced putative kinase 1, PTEN-induced putative kinase protein 1

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  WB analysis of HeLa using 23274-1-AP (same clone as 23274-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  WB analysis using 23274-1-AP (same clone as 23274-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  WB analysis using 23274-1-AP (same clone as 23274-1-PBS)

  Non-treated and CCCP treated HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 23274-1-AP (same clone as 23274-1-PBS)

  WB result of PINK1 antibody (23274-1-AP, 1:600) with si-Control and si-PINK1 transfected HeLa cells. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

23274-1-PBS bindet in WB, IHC, IF-P, ELISA PINK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PINK1 fusion protein Ag19825
• Vollständiger Name: PTEN induced putative kinase 1
• Berechnetes Molekulargewicht: 581 aa, 63 kDa
• Beobachtetes Molekulargewicht: 65 kDa, 45 kDa
• GenBank-Zugangsnummer: BC028215
• Gene symbol: PINK1
• Gene ID (NCBI): 65018
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.