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PINK1 Rekombinanter Antikörper

PINK1 Rekombinant Antikörper für WB, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240449E7

Kat-Nr. : 81991-4-PBS

Synonyme

240449E7, BRPK, EC:2.7.11.1, PARK6, PTEN induced putative kinase 1

Filter:
  • Western Blot (WB) analysis of various lysates using PINK1 Recombinant antibody (81991-4-RR)

    WB analysis using 81991-4-RR (same clone as 81991-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81991-4-RR (PINK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81991-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using PINK1 Recombinant antibody (81991-4-RR)

    FC experiment of Jurkat using 81991-4-RR (same clone as 81991-4-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PINK1 Recombinant antibody (81991-4-RR, Clone:240449E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81991-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81991-4-RR

    Affinity and Kinetic Characterization of 81991-4-RR

    Biolayer interferometry (BLl) kinetic assays of 81991-4-RR against Human PINK1 were performed. The affinity constant is 125 nM.

Geprüfte Anwendungen

Produktinformation

81991-4-PBS bindet in WB, FC (Intra), Cytometric bead array, Indirect ELISA PINK1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PINK1 fusion protein Ag19825
Vollständiger Name PTEN induced putative kinase 1
Berechnetes Molekulargewicht 581 aa, 63 kDa
GenBank-ZugangsnummerBC028215
Gene symbol PINK1
Gene ID (NCBI) 65018
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.