PIP4K2A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PIP4K2A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12469-1-PBS

Synonyme

Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, Phosphatidylinositol 5-Phosphate 4-Kinase, EC:2.7.1.149, Diphosphoinositide kinase 2-alpha

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• 

  WB analysis of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12469-1-AP (same clone as 12469-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12469-1-AP (same clone as 12469-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)

  IP result of anti-PIP4K2A (IP:12469-1-AP, 4ug; Detection:12469-1-AP 1:500) with mouse brain tissue lysate 4400ug. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 12469-1-AP (same clone as 12469-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 12469-1-AP (same clone as 12469-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12469-1-AP (PIP4K2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12469-1-AP (PIP4K2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12469-1-AP (same clone as 12469-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIP4K2A antibody (12469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (black). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 12469-1-AP (same clone as 12469-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 12469-1-AP (PIP4K2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12469-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA PIP4K2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIP4K2A fusion protein Ag3152
• Vollständiger Name: phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
• Berechnetes Molekulargewicht: 406 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC018034
• Gene symbol: PIP4K2A
• Gene ID (NCBI): 5305
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.