PIP4K2B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PIP4K2B Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 13218-1-AP

Synonyme

PI(5)P 4 kinase type II beta, PI5P4KB, PIP4K2B, PIP4KII beta, PIP5K2B, PIP5KIIB, PIP5KIIbeta

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  WB analysis of HeLa using 13218-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13218-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13218-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13218-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13218-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13218-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 13218-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13218-1-AP (PIP4K2B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 13218-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13218-1-AP (PIP4K2B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 13218-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13218-1-AP (PIP4K2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13218-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13218-1-AP (PIP4K2B Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

13218-1-AP bindet in WB, IHC, ELISA PIP4K2B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIP4K2B fusion protein Ag4099
• Vollständiger Name: phosphatidylinositol-5-phosphate 4-kinase, type II, beta
• Berechnetes Molekulargewicht: 416 aa, 47 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC027459
• Gene symbol: PIP4K2B
• Gene ID (NCBI): 8396
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIP4K2B, also named as PIP5K2B, participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. PIP4K2B has a homodimer. It binds TNFRSF1A(24/32/50kd).

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; MANF regulates hypothalamic control of food intake and body weight.; Authors - Su Yang ; KD Validated
mouse	IHC	Dev Cell; PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay.; Authors - Archna Ravi
human	WB	JBMR Plus; Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament; Authors - Hideaki Nakajima
human	WB	Eur J Med Chem; Design, synthesis and biological evaluation of the activity-based probes for FGFR covalent inhibitor; Authors - Dandan Zhu