PIP5K1B Polyklonaler Antikörper

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• KD/KO Validated

PIP5K1B Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 12541-1-AP

Synonyme

PIP5K1 beta, PIP5K1B, PIP5KIbeta, Protein STM 7, PtdIns(4)P 5 kinase 1 beta, STM7

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  WB analysis of human testis using 12541-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12541-1-AP (PIP5K1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12541-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12541-1-AP (PIP5K1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12541-1-AP

  IP result of anti-PIP5K1B (IP:12541-1-AP, 4ug; Detection:12541-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human lung using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, humanes Hirngewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Herzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

12541-1-AP bindet in WB, IP, IHC, ELISA PIP5K1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIP5K1B fusion protein Ag3261
• Vollständiger Name: phosphatidylinositol-4-phosphate 5-kinase, type I, beta
• Berechnetes Molekulargewicht: 549 aa, 62 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC030587
• Gene symbol: PIP5K1B
• Gene ID (NCBI): 8395
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Clin Invest; Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation.; Authors - Jaerak Chang
mouse	WB	EMBO J; PTPRN2 and PLCβ1 promote metastatic breast cancer cell migration through PI(4,5)P2-dependent actin remodeling.; Authors - Caitlin A Sengelaub
human	WB	EMBO J; Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKHM1 membrane cycling during autophagosome-lysosome fusion.; Authors - Takashi Baba ; KD Validated
human	WB	Cell Chem Biol; Targeting the PI5P4K Lipid Kinase Family in Cancer Using Covalent Inhibitors.; Authors - Sindhu Carmen Sivakumaren
		EMBO J; Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKMH1 membrane cycling during autophagosome-lysosome fusion.; Authors - Takashi Baba