PIR Polyklonaler Antikörper

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PIR Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 10263-1-AP

Synonyme

Probable quercetinase, Probable quercetin 2,3-dioxygenase PIR, Pirin, EC:1.13.11.24

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  WB analysis of HepG2 using 10263-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10263-1-AP (PIR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10263-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10263-1-AP (PIR antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10263-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10263-1-AP (PIR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10263-1-AP

  IP result of anti-PIR (IP:10263-1-AP, 4ug; Detection:10263-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IHC staining of human stomach using 10263-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10263-1-AP (PIR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

10263-1-AP bindet in WB, IHC, IP, ELISA PIR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIR fusion protein Ag0330
• Vollständiger Name: pirin (iron-binding nuclear protein)
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC002517
• Gene symbol: PIR
• Gene ID (NCBI): 8544
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pirin (iron-binding nuclear protein, PIR) is a highly conserved 32-kDa protein consisting of 290 amino acids. Pirin mRNA is expressed weakly in all human tissues. Confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Pirin interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and nuclear factor I (NFI).

Publikationen

Species	Application	Title
human	WB	Cell Cycle; PIR promotes tumorigenesis of breast cancer by upregulating cell cycle activator E2F1.; Authors - Muhammad Suleman ; KD Validated

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