PIR Monoklonaler Antikörper

PIR Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A10E4

Kat-Nr. : 67516-1-PBS

Synonyme

PIR, Pirin

---

• 

  WB analysis of HSC-T6 using 67516-1-Ig (same clone as 67516-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67516-1-Ig (PIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67516-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67516-1-Ig (same clone as 67516-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67516-1-Ig (PIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67516-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67516-1-Ig (same clone as 67516-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67516-1-Ig (PIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67516-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67516-1-Ig (same clone as 67516-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67516-1-Ig (PIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67516-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67516-1-PBS bindet in WB, Indirect ELISA PIR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PIR fusion protein Ag29969
• Vollständiger Name: pirin (iron-binding nuclear protein)
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC002517
• Gene symbol: PIR
• Gene ID (NCBI): 8544
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pirin (iron-binding nuclear protein, PIR) is a highly conserved 32-kDa protein consisting of 290 amino acids. Pirin mRNA is expressed weakly in all human tissues. Confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Pirin interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and nuclear factor I (NFI).