PISD Rekombinanter Antikörper

PISD Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242280C9

Kat-Nr. : 84866-2-PBS

Synonyme

242280C9, EC:4.1.1.65, Phosphatidylserine decarboxylase alpha chain, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase proenzyme, mitochondrial

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  WB analysis using 84866-2-RR (same clone as 84866-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84866-2-RR (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84866-2-PBS in a different storage buffer formulation.

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  WB analysis using 84866-2-RR (same clone as 84866-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84866-2-RR (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84866-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01640-2

  Sandwich ELISA standard curve of MP01640-2, Human PISD Recombinant Matched Antibody Pair - PBS only. 84866-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9499. 84866-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

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  Sample test of MP01640-2

  The mean PISD concentration was determined to be 19.97 ng/mL in L02 cell extract based on a 1.30 mg/mL extract load.

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  Affinity and Kinetic Characterization of 84866-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84866-2-RR against Human PISD were performed. The affinity constant is 0.441 nM.

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Geprüfte Anwendungen

Produktinformation

84866-2-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test PISD und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PISD fusion protein Ag9499
• Vollständiger Name: phosphatidylserine decarboxylase
• Berechnetes Molekulargewicht: 375aa,43 kDa; 408aa,46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC001482
• Gene symbol: PISD
• Gene ID (NCBI): 23761
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.