PITX2 Monoklonaler Antikörper

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• KD/KO Validated

PITX2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

1E3B1

Rezensionen (1)

Kat-Nr. : 67201-1-Ig

Synonyme

1E3B1, ALL1-responsive protein ARP1, ARP1, Paired-like homeodomain transcription factor 2, RIEG bicoid-related homeobox transcription factor

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• 

  WB analysis using 67201-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67201-1-Ig (PITX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis of HEK-293 using 67201-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67201-1-Ig (PITX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, HEK-293-Zellen, Maushirngewebe, Maus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

67201-1-Ig bindet in WB, IF, ELISA PITX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PITX2 fusion protein Ag27924
• Vollständiger Name: paired-like homeodomain 2
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC013998
• Gene symbol: PITX2
• Gene ID (NCBI): 5308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PITX2 belongs to the bicoid class of homeodomain transcription factors, which play essential roles in embryonic development. Various PITX2 isoforms are involved in a wide variety of developmental programs, including left-right signaling. It controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, PITX2 is involved in the expansion of muscle progenitors. In addition, it may play a role in the proper localization of asymmetric organs such as the heart and stomach. PITX2 can be bound by some other proteins, and are part of a much larger atrial transcriptional complex. This antibody specifically recognizes isoform PTX2C.

Publikationen

Species	Application	Title
rat	WB	Oral Dis; Enamel defects of Axenfeld-Rieger syndrome and the role of PITX2 in its pathogenesis.; Authors - Yi Yang ; KD Validated
mouse	WB	Aging (Albany NY); D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism; Authors - Heng Zhang ; KD Validated
mouse	IF	Genomics Proteomics Bioinformatics; Single-cell Atlas of Developing Mouse Palates Reveals Cellular and Molecular Transitions in Periderm Cell Fate; Authors - Wenbin Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (03-28-2024)

Worked well for immunofluorescence in mouse heart tissues.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse heart