PIWIL1 Polyklonaler Antikörper

PIWIL1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 15659-1-AP

Synonyme

Piwi like protein 1, piwi like 1 (Drosophila), PIWI, MIWI, HIWI

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  WB analysis of human testis using 15659-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15659-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15659-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 15659-1-AP

  IP result of anti-PIWIL1 (IP:15659-1-AP, 4ug; Detection:15659-1-AP 1:2000) with mouse testis tissue lysate 2400 ug.

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  IHC staining of human testis using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse testis using 15659-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using PIWIL1 antibody (15659-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15659-1-AP

  Immunofluorescent analysis of HepG2 cells using 15659-1-AP (PIWIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Leberkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

15659-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA PIWIL1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIWIL1 fusion protein Ag8056
• Vollständiger Name: piwi-like 1 (Drosophila)
• Berechnetes Molekulargewicht: 99 kDa
• Beobachtetes Molekulargewicht: 85-95 kDa
• GenBank-Zugangsnummer: BC028581
• Gene symbol: PIWIL1
• Gene ID (NCBI): 9271
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis; Authors -
human	WB	Mol Ther Nucleic Acids; PIWIL1/piRNA-DQ593109 Regulates the Permeability of the Blood-Tumor Barrier via the MEG3/miR-330-5p/RUNX3 Axis.; Authors - Shuyuan Shen
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.; Authors - Xiaoli Wang
human	WB	J Transl Med; piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner.; Authors - Qi Xie
human	IF	Prostate; High levels of PIWI-interacting RNAs are present in the small RNA landscape of prostate epithelium from vitamin D clinical trial specimens.; Authors - Bethany Baumann
mouse	WB	Andrologia; Secretions released from mesenchymal stem cells improve spermatogenesis restoration of cytotoxic treatment with busulfan in azoospermia mice.; Authors - Yi-Ting Cai