PJA1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PJA1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 17687-1-AP

Synonyme

E3 ubiquitin-protein ligase Praja-1, EC:2.3.2.27, praja ring finger 1, Praja1, RING finger protein 70

---

• 

  WB analysis using 17687-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17687-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17687-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17687-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17687-1-AP

  IP result of anti-PJA1 (IP:17687-1-AP, 3ug; Detection:17687-1-AP 1:300) with mouse brain tissue lysate 3600ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

17687-1-AP bindet in WB, IF, IP, CoIP, ELISA PJA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PJA1 fusion protein Ag11820
• Vollständiger Name: praja ring finger 1
• Berechnetes Molekulargewicht: 643 aa, 71 kDa
• Beobachtetes Molekulargewicht: 71-80 kDa
• GenBank-Zugangsnummer: BC075803
• Gene symbol: PJA1
• Gene ID (NCBI): 64219
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PJA1(E3 ubiquitin-protein ligase Praja-1), also named as RNF70, regulates the level of PRC2 (polycomb repressive complex 2) by targeting its free subunits for Ub-mediated proteasomal degradation and it can be involved in protein ubiquitination in the brain and is a suitable candidate gene for MRX(PMID:12036302). The Praja1 protein is unlikely to be a membrane receptor, since it lacks a hydrophobic transmembrane domain(PMID:9393880). It has 3 isoforms produced by alternative splicing with the molecular weight of 71 kDa, 50 kDa and 65 kDa.

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Commun; Praja1 E3 ubiquitin ligase promotes skeletal myogenesis through degradation of EZH2 upon p38α activation.; Authors - Silvia Consalvi ; KD Validated
human	WB	Ann Clin Transl Neurol; A recurrent PJA1 variant in trigonocephaly and neurodevelopmental disorders.; Authors - Toshimitsu Suzuki
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Diacylglycerol kinase δ destabilizes serotonin transporter protein through the ubiquitin-proteasome system.; Authors - Qiang Lu
human	WB	J Virol; PJA1 Coordinates with the SMC5/6 Complex to Restrict DNA Viruses and Episomal Genes through Interferon-independent Manner.; Authors - Wei Xu
rat	WB	Neuropathology; Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation.; Authors - Kazuhiko Watabe
rat	CoIP	Neuropathology; Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation.; Authors - Kazuhiko Watabe