PJA1 Polyklonaler Antikörper

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PJA1 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17687-1-PBS

Synonyme

E3 ubiquitin-protein ligase Praja-1, EC:2.3.2.27, praja ring finger 1, Praja1, RING finger protein 70

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  WB analysis using 17687-1-AP (same clone as 17687-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 17687-1-AP (same clone as 17687-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

  IP result of anti-PJA1 (IP:17687-1-AP, 3ug; Detection:17687-1-AP 1:300) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17687-1-PBS bindet in WB, IP, Indirect ELISA PJA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PJA1 fusion protein Ag11820
• Vollständiger Name: praja ring finger 1
• Berechnetes Molekulargewicht: 643 aa, 71 kDa
• Beobachtetes Molekulargewicht: 71-80 kDa
• GenBank-Zugangsnummer: BC075803
• Gene symbol: PJA1
• Gene ID (NCBI): 64219
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PJA1(E3 ubiquitin-protein ligase Praja-1), also named as RNF70, regulates the level of PRC2 (polycomb repressive complex 2) by targeting its free subunits for Ub-mediated proteasomal degradation and it can be involved in protein ubiquitination in the brain and is a suitable candidate gene for MRX(PMID:12036302). The Praja1 protein is unlikely to be a membrane receptor, since it lacks a hydrophobic transmembrane domain(PMID:9393880). It has 3 isoforms produced by alternative splicing with the molecular weight of 71 kDa, 50 kDa and 65 kDa.