PKC Delta Polyklonaler Antikörper

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PKC Delta Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19132-1-PBS

Synonyme

PRKCD, EC:2.7.10.2, EC:2.7.11.13, MAY1, nPKC-delta

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  WB analysis using 19132-1-AP (same clone as 19132-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC Delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  WB analysis using 19132-1-AP (same clone as 19132-1-PBS)

  Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 19132-1-AP (same clone as 19132-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 19132-1-AP (same clone as 19132-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 19132-1-AP (same clone as 19132-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 19132-1-AP (same clone as 19132-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-1080 using 19132-1-AP (same clone as 19132-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 19132-1-AP (same clone as 19132-1-PBS)

  IP result of anti-PKC Delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:800) with A431 cells lysate 2640 ug. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 19132-1-AP (same clone as 19132-1-PBS)

  IP result of anti-PKC Delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:500) with A431 cells lysate 2000ug. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 19132-1-AP (same clone as 19132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19132-1-AP (PKC Delta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 19132-1-AP (same clone as 19132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19132-1-AP (PKC Delta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 19132-1-AP (same clone as 19132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 19132-1-AP (PKC delta antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 19132-1-AP (same clone as 19132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 19132-1-AP (PKC delta antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 19132-1-AP (same clone as 19132-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 19132-1-AP (same clone as 19132-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19132-1-AP (same clone as 19132-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 19132-1-AP (same clone as 19132-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 19132-1-AP (same clone as 19132-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Delta (19132-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19132-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA PKC Delta und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PKC Delta fusion protein Ag5631
• Vollständiger Name: protein kinase C, delta
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC043350
• Gene symbol: PKC Delta
• Gene ID (NCBI): 5580
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.