PKC Gamma Polyklonaler Antikörper

PKC Gamma Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29208-1-PBS

Synonyme

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  WB analysis using 29208-1-AP (same clone as 29208-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29208-1-AP (PKC Gamma antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29208-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 29208-1-AP (same clone as 29208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29208-1-AP (PKC Gamma antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29208-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 29208-1-AP (same clone as 29208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29208-1-AP (PKC Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29208-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29208-1-PBS bindet in WB, IHC, Indirect ELISA PKC Gamma und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PKC Gamma fusion protein Ag25085
• Vollständiger Name: protein kinase C, gamma
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC047876
• Gene symbol: PKC Gamma
• Gene ID (NCBI): 5582
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.