• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

PKC Iota Monoklonaler Antikörper

PKC Iota Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A1B6

Kat-Nr. : 66493-1-PBS

Synonyme

PRKCI, 3A1B6, aPKC lambda/iota, aPKC-lambda/iota, Atypical protein kinase C-lambda/iota

Filter:
  • Western Blot (WB) analysis of various lysates using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis using 66493-1-Ig (same clone as 66493-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC Iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of HeLa using 66493-1-Ig (same clone as 66493-1-PBS)

    WB result of PKC Iota antibody (66493-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Iota transfected HeLa cells. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis using 66493-1-Ig (same clone as 66493-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of MCF-7 using 66493-1-Ig (same clone as 66493-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of HEK-293 using 66493-1-Ig (same clone as 66493-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of ROS1728 using 66493-1-Ig (same clone as 66493-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of C6 using 66493-1-Ig (same clone as 66493-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PKC Iota Monoclonal antibody (66493-1-Ig)

    IHC staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PKC Iota Monoclonal antibody (66493-1-Ig)

    IHC staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using PKC Iota Monoclonal antibody (66493-1-Ig)

    IF Staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    IF Staining of HeLa using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66493-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA PKC Iota und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen PKC Iota fusion protein Ag4990
Vollständiger Name protein kinase C, iota
Berechnetes Molekulargewicht 68 kDa
Beobachtetes Molekulargewicht 70 kDa
GenBank-ZugangsnummerBC022016
Gene symbol PKC Iota
Gene ID (NCBI) 5584
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.