PKC Iota Rekombinanter Antikörper

PKC Iota Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

243126A4

Kat-Nr. : 85733-2-PBS

Synonyme

PRKCI, aPKC lambda/iota, aPKC-lambda/iota, Atypical protein kinase C-lambda/iota, DXS1179E

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  WB analysis using 85733-2-RR (same clone as 85733-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85733-2-RR (PRKCI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85733-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02074-3

  Sandwich ELISA standard curve of MP02074-3, Human PKC Iota Recombinant Matched Antibody Pair - PBS only. 85733-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4990. 85733-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP02074-3

  The mean PKC Iota concentration was determined to be 47.20 ng/mL in HeLa cell extract based on a 3.0 mg/mL extract load.

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  Affinity and Kinetic Characterization of 85733-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85733-2-RR against Human PKC Iota were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

85733-2-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test PKC Iota und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PKC Iota fusion protein Ag4990
• Vollständiger Name: protein kinase C, iota
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC022016
• Gene symbol: PKC Iota
• Gene ID (NCBI): 5584
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.