PKIA Polyklonaler Antikörper

PKIA Polyklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11743-1-PBS

Synonyme

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  IHC staining of mouse embryo using 11743-1-AP (same clone as 11743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11743-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 11743-1-AP (same clone as 11743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11743-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11743-1-AP (same clone as 11743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11743-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11743-1-AP (same clone as 11743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11743-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11743-1-AP (same clone as 11743-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PKIA antibody (11743-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11743-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11743-1-PBS bindet in IHC, IF/ICC, Indirect ELISA PKIA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PKIA fusion protein Ag2338
• Vollständiger Name: protein kinase (cAMP-dependent, catalytic) inhibitor alpha
• Berechnetes Molekulargewicht: 76 aa, 8 kDa
• GenBank-Zugangsnummer: BC022265
• Gene symbol: PKIA
• Gene ID (NCBI): 5569
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

cAMP-dependent protein kinase inhibitor alpha (PKIA) is also named as PRKACN1, and belongs to the PKI family. Among them, PKIA, a member of protein kinase A (PKA) inhibitor family, was found to be most significantly and highly expressed in susceptible animals (PMID:24910983). Decreased PKIA signaling would directly impact PKA activation, potentially inducing DRP1 phosphorylation and increasing ER Ca2+ stores (PMID:32152556). miR-129-5p activated PKA to regulate the phosphorylation of beta-catenin and cAMP-response element binding protein (CREB) by inhibiting PKIA (PMID:36222334).