PKLR Rekombinanter Antikörper

PKLR Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251231F12

Kat-Nr. : 86485-1-PBS

Synonyme

EC:2.7.1.40, PK1, PKL, Pyruvate kinase isozymes L/R, Pyruvate kinase PKLR

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  WB analysis of HepG2 using 86485-1-RR (same clone as 86485-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 86485-1-RR (PKLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86485-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 86485-1-RR (same clone as 86485-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 86485-1-RR (PKLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86485-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02639-1

  The mean PKLR concentration was determined to be 113.54 ng/mL in NIH/3T3 cell extract based on a 1.20 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02639-1

  Sandwich ELISA standard curve of MP02639-1, Human PKLR Recombinant Matched Antibody Pair - PBS only. 86485-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag17933. 86485-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 86485-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86485-1-RR against Human PKLR were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

86485-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA PKLR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PKLR fusion protein Ag17933
• Vollständiger Name: pyruvate kinase, liver and RBC
• Berechnetes Molekulargewicht: 574 aa, 62 kDa
• Beobachtetes Molekulargewicht: 58-62 kDa
• GenBank-Zugangsnummer: BC025737
• Gene symbol: PKLR
• Gene ID (NCBI): 5313
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKLR(Pyruvate kinase isozymes R/L) is also named as PK1,PKL,which is a glycolytic enzyme that catalyzes the transphosphorylation from phosphoenolpyruvate (PEP) to ADP, yielding pyruvate and ATP. It is the last step of the glycolytic pathway and is essentially irreversible.It belongs to the pyruvate kinase family and There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) and pyruvate kinase deficiency of red cells (PKRD).It can form a homotetramer(PMID:11960989).