PKM2-specific Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PKM2-specific Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C11C7

Kat-Nr. : 60268-1-PBS

Synonyme

PKM2, PK-M2, PKM2 pyruvate kinase, muscle, PKM, Pyruvate kinase PKM

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  WB analysis of HEK-293 using 60268-1-Ig (same clone as 60268-1-PBS)

  WB result of PKM2 antibody (60268-1-Ig, 1:3000) with si-Control and si-PKM2 transfected HEK293 cells. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis using 60268-1-Ig (same clone as 60268-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60268-1-Ig (same clone as 60268-1-PBS)

  Western blot analysis of PK-M2-specific in various lines using Proteintech antibody 60268-1-Ig at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 60268-1-Ig (same clone as 60268-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 60268-1-Ig (same clone as 60268-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60268-1-Ig (same clone as 60268-1-PBS)

  IP result of anti-PKM2-specific (IP:60268-1-Ig, 5ug; Detection:15822-1-AP (PKM2-specific rabbit poly-antibody), 1:300) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:0 (under 10x lens). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:0 (under 40x lens). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKM2-specific antibody (60268-1-Ig, Clone: 1C11C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60268-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA PKM2-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: PKM pyruvate kinase, muscle
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: NM_002654
• Gene symbol: PKM
• Gene ID (NCBI): 5315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms,the activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.