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PKMYT1 Polyklonaler Antikörper

PKMYT1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29489-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using PKMYT1 Polyclonal antibody (29489-1-AP)

    WB analysis of MCF-7 using 29489-1-AP (same clone as 29489-1-PBS)

    WB result of PKMYT1 antibody (29489-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKMYT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 29489-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PKMYT1 Polyclonal antibody (29489-1-AP)

    WB analysis using 29489-1-AP (same clone as 29489-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29489-1-AP (PKMYT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29489-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29489-1-PBS bindet in WB, Indirect ELISA PKMYT1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PKMYT1 fusion protein Ag30573
Vollständiger Name protein kinase, membrane associated tyrosine/threonine 1
Berechnetes Molekulargewicht 55 kDa
Beobachtetes Molekulargewicht60-75 kDa
GenBank-ZugangsnummerNM_004203
Gene symbol PKMYT1
Gene ID (NCBI) 9088
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKMYT1, also known as MYT1, belongs to the WEE1 family. PKMYT1 functions to negatively regulate the Cdc2-cyclin B complexes by phosphorylating Cdc2 on threonine 14 and tyrosine 15. PKMYT1 has also been implicated in an increasing number of cancer types, including gastric cancer, non-small-cell lung cancer, hepatocellular carcinoma, glioblastoma, neuroblastoma, and colorectal cancer, in which overexpression of PKMYT1 generally correlates with poor prognosis and disease progression. PKMYT1 also has another key role in the cell cycle - regulating Golgi membrane reassembly following mitosis - and depletion of PKMYT1 leads to cell death. PKMYT1 was strongly phosphorylated during mitosis. This antibody can recognize interphase and mitotic phase PKMYT1. (PMID: 9268380, 32127662)