PKNOX2 Polyklonaler Antikörper

PKNOX2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, RIP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20352-1-AP

Synonyme

Homeobox protein PKNOX2, Homeobox protein PREP 2, PBX/knotted 1 homeobox 2, PBX/knotted homeobox 2, PKNOX2, PREP2

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Galerie der Validierungsdaten

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  WB analysis of RAW264.7 using 20352-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 20352-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20352-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20352-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 20352-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 20352-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20352-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20352-1-AP (PKNOX2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20352-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20352-1-AP (PKNOX2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, RAW264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Produktinformation

20352-1-AP bindet in WB, RIP, IHC, IF, ELISA PKNOX2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PKNOX2 fusion protein Ag14195
• Vollständiger Name: PBX/knotted 1 homeobox 2
• Berechnetes Molekulargewicht: 472 aa, 52 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 55 kDa
• GenBank-Zugangsnummer: BC045626
• Gene symbol: PKNOX2
• Gene ID (NCBI): 63876
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKNOX2, PBX/knotted 1 homeobox 2, also named as PREP2, belongs to the three-amino acid loop extension (TALE) homeobox family. As it contains a DNA-binding motif, PKNOX2 may function as a nuclear transcription factor, experiments have reported the PKNOX2 protein locates to the nucleus. It was showed that highest expression of a 4-kb PREP2 (PKNOX2) transcript in heart, brain, skeletal muscle, and ovary(PMID:11972344). Several years later, PKNOX2 was identified as one of the cis-regulated genes for alcohol addiction in mice, however it has not been reported to be associated with any similar phenotype in humans to date (PMID:19721000). The molecular weight of the protein may differ from the theoretical value.

Publikationen

Species	Application	Title
mouse	IF	Biochem Biophys Res Commun; PKNOX2 regulates myofibroblast functions and tubular cell survival during kidney fibrosis.; Authors - Yoshiaki Miyake
pig	WB,RIP	Int J Mol Sci; LncRNA TCONS_00323213 Promotes Myogenic Differentiation by Interacting with PKNOX2 to Upregulate MyoG in Porcine Satellite Cells; Authors - Mengxun Li