PKNOX2 Polyklonaler Antikörper

PKNOX2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20352-1-PBS

Synonyme

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  WB analysis of RAW264.7 using 20352-1-AP (same clone as 20352-1-PBS)

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse small intestine using 20352-1-AP (same clone as 20352-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 20352-1-AP (same clone as 20352-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 20352-1-AP (same clone as 20352-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 20352-1-AP (same clone as 20352-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 20352-1-AP (same clone as 20352-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20352-1-AP (same clone as 20352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20352-1-AP (PKNOX2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20352-1-AP (same clone as 20352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20352-1-AP (PKNOX2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20352-1-PBS bindet in WB, IHC, Indirect ELISA PKNOX2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PKNOX2 fusion protein Ag14195
• Vollständiger Name: PBX/knotted 1 homeobox 2
• Berechnetes Molekulargewicht: 472 aa, 52 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 55 kDa
• GenBank-Zugangsnummer: BC045626
• Gene symbol: PKNOX2
• Gene ID (NCBI): 63876
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKNOX2, PBX/knotted 1 homeobox 2, also named as PREP2, belongs to the three-amino acid loop extension (TALE) homeobox family. As it contains a DNA-binding motif, PKNOX2 may function as a nuclear transcription factor, experiments have reported the PKNOX2 protein locates to the nucleus. It was showed that highest expression of a 4-kb PREP2 (PKNOX2) transcript in heart, brain, skeletal muscle, and ovary(PMID:11972344). Several years later, PKNOX2 was identified as one of the cis-regulated genes for alcohol addiction in mice, however it has not been reported to be associated with any similar phenotype in humans to date (PMID:19721000). The molecular weight of the protein may differ from the theoretical value.