Plakophilin 3 Polyklonaler Antikörper

Plakophilin 3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 18338-1-AP

Synonyme

PKP3, Plakophilin-3

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  WB analysis of K-562 using 18338-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18338-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 18338-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18338-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 18338-1-AP

  IP result of anti-Plakophilin 3 (IP:18338-1-AP, 4ug; Detection:18338-1-AP 1:600) with A549 cells lysate 1000ug.

• 

  IHC staining of human skin using 18338-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18338-1-AP (Plakophilin 3 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 18338-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18338-1-AP (Plakophilin 3 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse skin using 18338-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 18338-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 18338-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 18338-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18338-1-AP (Plakophilin 3 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 18338-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18338-1-AP (Plakophilin 3 antibody at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, A549-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Kolongewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 3 publications below

Produktinformation

18338-1-AP bindet in WB, IHC, IP, ELISA Plakophilin 3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Plakophilin 3 fusion protein Ag13062
• Vollständiger Name: plakophilin 3
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 87 kDa
• GenBank-Zugangsnummer: BC000081
• Gene symbol: Plakophilin 3
• Gene ID (NCBI): 11187
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB,IHC	J Ovarian Res; Cangfu Daotan Wan alleviates polycystic ovary syndrome with phlegm-dampness syndrome via disruption of the PKP3/ERCC1/MAPK axis; Authors - Yuan Li
human	IHC	Biomedicines; Comprehensive Analysis Identifies PKP3 Overexpression in Pancreatic Cancer Related to Unfavorable Prognosis; Authors - Yan Du
human	IHC	Med Oncol; Data-independent acquisition (DIA) mass spectrometry reveals related proteins involved in the occurrence of early intestinal-type gastric cancer; Authors - Liangshun Zhang