PLA2G12B Polyklonaler Antikörper

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• KD/KO Validated

PLA2G12B Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25799-1-AP

Synonyme

FKSG71, Group XIIB secretory phospholipase A2-like protein, Group XIII secretory phospholipase A2-like protein, GXIIB, GXIII sPLA2 like

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  WB analysis of HepG2 using 25799-1-AP

  WB result of PLA2G12B antibody (25799-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLA2G12B transfected HepG2 cells.

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  WB analysis of HuH-7 using 25799-1-AP

  WB result of PLA2G12B antibody (25799-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLA2G12B transfected HuH-7 cells.

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  WB analysis using 25799-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25799-1-AP (PLA2G12B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 25799-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25799-1-AP (PLA2G12B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen, HuH-7-Zellen, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25799-1-AP bindet in WB, ELISA PLA2G12B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLA2G12B fusion protein Ag22877
• Vollständiger Name: phospholipase A2, group XIIB
• Berechnetes Molekulargewicht: 195 aa, 22 kDa
• Beobachtetes Molekulargewicht: 21-22 kDa
• GenBank-Zugangsnummer: BC111946
• Gene symbol: PLA2G12B
• Gene ID (NCBI): 84647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phospholipase A2 (PLA2) enzymes catalyze hydrolysis of glycolipids to release free fatty acids and lysophospholipids. PLA2G12B is a secreted group XIIB secretory phospholipase A2-like protein belonging to the PLA2 family, but it is catalytically inactive due to an amino acid change in its active site and has altered phospholipid-binding properties (PMID: 14516201). It is located outside the cell membranes and strongly expressed in liver, small intestine and kidney, and recently reports revealed that Pla2g12b gene may be implicated in HDL cholesterol levels and metabolism (PMID: 22912808). The calculated molecular weight of PLA2G12B is 21 kDa.