PLA2G1B Polyklonaler Antikörper

PLA2G1B Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15843-1-PBS

Synonyme

PLA2, Phosphatidylcholine 2-acylhydrolase 1B, EC:3.1.1.4

---

• 

  WB analysis using 15843-1-AP (same clone as 15843-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15843-1-AP (same clone as 15843-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 15843-1-AP (same clone as 15843-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 15843-1-AP (same clone as 15843-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15843-1-AP (PLA2G1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 15843-1-AP (same clone as 15843-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15843-1-AP (PLA2G1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 15843-1-AP (same clone as 15843-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using PLA2G1B antibody (15843-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

15843-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA PLA2G1B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLA2G1B fusion protein Ag8603
• Vollständiger Name: phospholipase A2, group IB (pancreas)
• Berechnetes Molekulargewicht: 70aa,8 kDa; 148aa,16 kDa
• Beobachtetes Molekulargewicht: 13-16 kDa
• GenBank-Zugangsnummer: BC005386
• Gene symbol: PLA2G1B
• Gene ID (NCBI): 5319
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLA2G1B is a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. PLA2G1B catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids.