PLA2G4D Polyklonaler Antikörper

PLA2G4D Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13345-1-AP

Synonyme

Cpla2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 13345-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13345-1-AP (PLA2G4D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 13345-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13345-1-AP (PLA2G4D Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 13345-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 13345-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 13345-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 13345-1-AP (PLA2G4D antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 13345-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 13345-1-AP (PLA2G4D antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin cancer using 13345-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 13345-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin cancer using 13345-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13345-1-AP bindet in WB, IHC,ELISA PLA2G4D und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLA2G4D fusion protein Ag4178
• Vollständiger Name: phospholipase A2, group IVD (cytosolic)
• Berechnetes Molekulargewicht: 818 aa, 92 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: BC034571
• Gene symbol: PLA2G4D
• Gene ID (NCBI): 283748
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560).