PLA2G4E Polyklonaler Antikörper

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• KD/KO Validated

PLA2G4E Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18088-1-PBS

Synonyme

phospholipase A2, group IVE, PLA2G4E Ens

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  WB analysis of SH-SY5Y using 18088-1-AP (same clone as 18088-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18088-1-AP (same clone as 18088-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

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  WB analysis of SW 1990 using 18088-1-AP (same clone as 18088-1-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 18088-1-AP (same clone as 18088-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 18088-1-AP (same clone as 18088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 18088-1-AP (same clone as 18088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 18088-1-AP (same clone as 18088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 18088-1-AP (same clone as 18088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18088-1-AP (same clone as 18088-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PLA2G4E antibody (18088-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18088-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PLA2G4E und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLA2G4E fusion protein Ag12808
• Vollständiger Name: phospholipase A2, group IVE
• Berechnetes Molekulargewicht: 868 aa, 99 kDa
• Beobachtetes Molekulargewicht: 99 kDa, 57 kDa
• GenBank-Zugangsnummer: BC101584
• Gene symbol: PLA2G4E
• Gene ID (NCBI): 123745
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLA2G4E (Phospholipase A2 group IVE), also known as cPLA2-epsilon. Calcium-dependent N-acyltransferase is involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain (PMID:29447909). Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide (PMID:29447909; 30517655).