PAFAH Polyklonaler Antikörper

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• KD/KO Validated

PAFAH Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 15526-1-AP

Synonyme

Group VIIA phospholipase A2, gVIIA PLA2, LDL PLA(2), LDL PLA2, Lp-PLA2, PAF 2 acylhydrolase, PAF acetylhydrolase, PAFAH, PAF-AH, PLA2G7

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Galerie der Validierungsdaten

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  WB analysis of RAW264.7 using 15526-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 15526-1-AP (PAFAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 15526-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 15526-1-AP (PAFAH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 15526-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15526-1-AP (PAFAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15526-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15526-1-AP (PAFAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 15526-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15526-1-AP (PAFAH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15526-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15526-1-AP (PAFAH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15526-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15526-1-AP (PAFAH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15526-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15526-1-AP (PAFAH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of RAW 264.7 using 15526-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed RAW 264.7 cells using 15526-1-AP (PAFAH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, Mauslebergewebe, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 2 publications below

Produktinformation

15526-1-AP bindet in WB, IHC, IF, ELISA PAFAH und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAFAH fusion protein Ag7227
• Vollständiger Name: phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 43-67 kDa
• GenBank-Zugangsnummer: BC038452
• Gene symbol: PLA2G7
• Gene ID (NCBI): 7941
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAFAH(platelet-activating factor acetylhydrolase, plasma) is also named as PLA2G7(phospholipase A2, group VII), Lp-PLA2 (Lipoprotein-associated phospholipase A2), LDL-PLA2, LDL-PLA and belongs to the AB hydrolase superfamily and lipase family. It is an enzyme which catalyzes the hydrolysis of acetyl ester at the sn-2 position of PAF. The family of PAF-Ahs consists of two intracellular isoforms (Ib and II), and one secreted isoform (plasma). Plasma PAF-AH is a monomeric polypeptide with a molecular weight of 45 kDa and it can show broad bands with a molecular weight of 43-67 kDa that it can be extensively N-glycosylated(PMID:12547653). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PAFAD), susceptibility to asthma (ASTHMA), susceptibility to atopic hypersensitivity (ATOPY).

Publikationen

Species	Application	Title
mouse	WB	Nat Cell Biol; Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation.; Authors - Das Amrita A
mouse	WB	Nat Commun; VDAC2 enables BAX to mediate apoptosis and limit tumor development.; Authors - Hui San Chin
mouse	WB	J Extracell Vesicles; Characterization of protein, long noncoding RNA and microRNA signatures in extracellular vesicles derived from resting and degranulated mast cells.; Authors - Yuting Liang
mouse	IF,WB	Nat Metab; Elevated dietary ω-6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditions.; Authors - Jacob T Boyd ; KD Validated
human	IF	Cell Death Discov; Characterizing cellular heterogeneity in fibrotic hypersensitivity pneumonitis by single-cell transcriptional analysis.; Authors - Junyi Wang
human	WB	Int J Mol Sci; PLA2G7/PAF-AH as Potential Negative Regulator of the Wnt Signaling Pathway Mediates Protective Effects in BRCA1 Mutant Breast Cancer; Authors - Yue Liao ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sathyanarayani (Verified Customer) (05-12-2023)

I used this PAFAH antibody at a range of different dilutions from 1:300 to 1:1000 but unfortunately I could not detect any bands from this antibody when I used lysed mouse blood. The loading controls which were also from Proteintech produced strong clear bands on the western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Lysed mouse blood