PLAA Polyklonaler Antikörper

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• KD/KO Validated

PLAA Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12529-1-PBS

Synonyme

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  WB analysis of MCF-7 using 12529-1-AP (same clone as 12529-1-PBS)

  WB result of PLAA antibody (12529-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12529-1-AP (same clone as 12529-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12529-1-AP (PLAA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 12529-1-AP (same clone as 12529-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12529-1-AP (PLAA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 12529-1-AP (same clone as 12529-1-PBS)

  IP result of anti-PLAA (IP:12529-1-AP, 4ug; Detection:12529-1-AP 1:600) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12529-1-AP (same clone as 12529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12529-1-AP (same clone as 12529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12529-1-AP (same clone as 12529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12529-1-AP (same clone as 12529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12529-1-PBS bindet in WB, IHC, IP, Indirect ELISA PLAA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLAA fusion protein Ag3224
• Vollständiger Name: phospholipase A2-activating protein
• Berechnetes Molekulargewicht: 738 aa, 81 kDa
• Beobachtetes Molekulargewicht: 72-74 kDa
• GenBank-Zugangsnummer: BC032551
• Gene symbol: PLAA
• Gene ID (NCBI): 9373
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.