PLAGL2 Monoklonaler Antikörper

PLAGL2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

3C2E5

Kat-Nr. : 68606-1-Ig

Synonyme

ZNF900, Pleiomorphic adenoma-like protein 2, KIAA0198, 3C2E5

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  WB analysis using 68606-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68606-1-Ig (PLAGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of rabbit testis using 68606-1-Ig

  rabbit testis tissue were subjected to SDS PAGE followed by western blot with 68606-1-Ig (PLAGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 68606-1-Ig

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PLAGL2 Monoclonal antibody (68606-1-Ig, Clone:3C2E5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, Kaninchenhodengewebe
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68606-1-Ig bindet in WB, ELISA PLAGL2 und zeigt Reaktivität mit human, Kaninchen

• Getestete Reaktivität: human, Kaninchen
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PLAGL2 fusion protein Ag30227
• Vollständiger Name: pleiomorphic adenoma gene-like 2
• Berechnetes Molekulargewicht: 496 aa, 55 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC023655
• Gene symbol: PLAGL2
• Gene ID (NCBI): 5326
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLAGL2, also named KIAA0198, belongs to the krueppel C2H2-type zinc-finger protein family. It shows weak transcriptional activatory activity. PLAGL2, a proto-oncogene, is implicated in a variety of cancers including acute myeloid leukemia (AML), malignant glioma, colon cancer, and lung adenocarcinoma. PLAGL2 can function as a tumor suppressor by initiating cell cycle arrest and apoptosis. The calculated molecular weight of PLAGL2 is 55 kDa, but the SUMO1-modifIed form is 68 kDa (PMID: 30535429).