PLAP Monoklonaler Antikörper

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PLAP Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B7C9

Kat-Nr. : 60294-1-PBS

Synonyme

PLAP-1, PALP, EC:3.1.3.1, ALPP, ALP

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  WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

  WB result of PLAP antibody (60294-1-Ig, 1:1000) with si-control and si-PLAP transfected HepG2 cells. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

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  WB analysis using 60294-1-Ig (same clone as 60294-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 10x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 40x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60294-1-PBS bindet in WB, IHC, Indirect ELISA PLAP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PLAP fusion protein Ag13361
• Vollständiger Name: alkaline phosphatase, placental (Regan isozyme)
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC009647
• Gene symbol: PLAP
• Gene ID (NCBI): 250
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.