uPAR/CD87 Polyklonaler Antikörper

uPAR/CD87 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(27)

Rezensionen (1)

Kat-Nr. : 10286-1-AP

Synonyme

PLAUR, CD87, MO3, U PAR, UPAR

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• 

  WB analysis using 10286-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10286-1-AP (uPAR/CD87 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 10286-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10286-1-AP (uPAR/CD87 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U-251 using 10286-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using uPAR/CD87 antibody (10286-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, A2780-Zellen, U-251-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 8 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Produktinformation

10286-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA uPAR/CD87 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: uPAR/CD87 fusion protein Ag0187
• Vollständiger Name: PLAUR
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 35-70 kDa
• GenBank-Zugangsnummer: BC002788
• Gene symbol: uPAR
• Gene ID (NCBI): 5329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

uPAR is a 45-65 kDa, highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodelling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)

Publikationen

Species	Application	Title
mouse	IF	Mol Cell; A transient transcriptional activation governs unpolarized-to-polarized morphogenesis during embryo implantation; Authors - Xuehui Lyu
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
mouse	WB	J Hazard Mater; Epigenetic reprogramming of HDAC2 in CA1 excitatory neurons determines Pb-induced non-spatial memory deficits; Authors - Mengmeng Wang
mouse	IHC	Cancer Res; Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer.; Authors - Yogev Sela
human	WB	Nucleic Acids Res; Regulation of u-PAR gene expression by H2A.Z is modulated by the MEK-ERK/AP-1 pathway.; Authors - Santosh Chauhan
human	IHC	Mech Ageing Dev; Induction of epithelial cell senescence by SERPINE1 derived from fibroblasts in the amnion at parturition; Authors - Li-Jun Ling

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ruchi (Verified Customer) (07-26-2022)

IF on mouse skin

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Mouse skin

uPAR/CD87 Antibody Immunofluorescence validation (1:50 dilution) in Mouse skin (Cat no:10286-1-AP)

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