uPAR Polyklonaler Antikörper

uPAR Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Rezensionen (1)

Kat-Nr. : 10286-1-AP

Synonyme

CD87, MO3, PLAUR, U PAR, UPAR, URKR

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Galerie der Validierungsdaten

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  WB analysis using 10286-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10286-1-AP (uPAR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 10286-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10286-1-AP (uPAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  FC experiment of U-937 using 10286-1-AP

  1X10^6 U-937 cells were stained with 0.2ug uPAR antibody (10286-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, A2780-Zellen, U-251-Zellen, U-937-Zellen
• Erfolgreiche Detektion in FC: U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

10286-1-AP bindet in WB, IHC, IF, FC, CoIP, ELISA uPAR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: uPAR fusion protein Ag0187
• Vollständiger Name: PLAUR
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 35-70 kDa
• GenBank-Zugangsnummer: BC002788
• Gene symbol: PLAUR
• Gene ID (NCBI): 5329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

uPAR is a 45-65 kDa, highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodelling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
mouse	IHC	Cancer Res; Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer.; Authors - Yogev Sela
human	WB	Nucleic Acids Res; Regulation of u-PAR gene expression by H2A.Z is modulated by the MEK-ERK/AP-1 pathway.; Authors - Santosh Chauhan
human,mouse	WB,IHC	FASEB J; CLEC14A protects against podocyte injury in mice with adriamycin nephropathy.; Authors - Zeyu Su
human	IHC	J Inflamm Res; PLAUR as a Potential Biomarker Associated with Immune Infiltration in Bladder Urothelial Carcinoma.; Authors - Mulin Liu
human	WB	Front Oncol; Forkhead Box Q1 Is Critical to Angiogenesis and Macrophage Recruitment of Colorectal Cancer.; Authors - Hui Tang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ruchi (Verified Customer) (07-26-2022)

IF on mouse skin

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Mouse skin

uPAR Antibody Immunofluorescence validation (1:50 dilution) in Mouse skin (Cat no:10286-1-AP)

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