PLCB3 Polyklonaler Antikörper

PLCB3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21370-1-PBS

Synonyme

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3, EC:3.1.4.11, Phosphoinositide phospholipase C-beta-3, Phospholipase C beta 3, Phospholipase C-beta-3

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  WB analysis of MCF-7 using 21370-1-AP (same clone as 21370-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21370-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 21370-1-AP (same clone as 21370-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21370-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 21370-1-AP (same clone as 21370-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21370-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 21370-1-AP (same clone as 21370-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21370-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 21370-1-AP (same clone as 21370-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21370-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 21370-1-AP (same clone as 21370-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21370-1-AP (PLCB3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21370-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 21370-1-AP (same clone as 21370-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21370-1-AP (PLCB3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21370-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21370-1-PBS bindet in WB, IHC, Indirect ELISA PLCB3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLCB3 fusion protein Ag15845
• Vollständiger Name: phospholipase C, beta 3 (phosphatidylinositol-specific)
• Berechnetes Molekulargewicht: 1234 aa, 139 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC142681
• Gene symbol: PLCB3
• Gene ID (NCBI): 5331
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.