PLCD3 Polyklonaler Antikörper

PLCD3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 16792-1-AP

Synonyme

Phospholipase C-delta-3, phospholipase C, delta 3, Phospholipase C delta 3, Phosphoinositide phospholipase C-delta-3, EC:3.1.4.11

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  WB analysis of SH-SY5Y using 16792-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 16792-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 16792-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 16792-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16792-1-AP (PLCD3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 16792-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16792-1-AP (PLCD3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, A549-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

16792-1-AP bindet in WB, IHC, ELISA PLCD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLCD3 fusion protein Ag10249
• Vollständiger Name: phospholipase C, delta 3
• Berechnetes Molekulargewicht: 789 aa, 89 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC072384
• Gene symbol: PLCD3
• Gene ID (NCBI): 113026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phospholipase C Delta 3 (PLCD3) is a protein-coding gene located on chromosome 17 and a member of the phospholipase C family. PLCD3 is expressed broadly across different tissues, with high expression levels observed in the colon and urinary bladder, among others. The enzyme localizes to the plasma membrane and requires calcium for activation. Spermine, sphingosine, and several phospholipids can inhibit its activity. Recent studies have implicated PLCD3 in various types of cancer. In summary, PLCD3 is an important enzyme involved in cellular signaling and has been implicated in the development and progression of various cancers, making it a potential target for therapeutic interventions.

Publikationen

Species	Application	Title
pig	WB	J Cell Biochem; Phospholipase C inhibits apoptosis of porcine oocytes cultured in vitro.; Authors - Hua Li Chen
human	WB	Oncol Rep; PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells.; Authors - Weidong Liu