PLCG1 Rekombinanter Antikörper

PLCG1 Rekombinant Antikörper für WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242529A11

Kat-Nr. : 84941-1-PBS

Synonyme

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, 242529A11, EC:3.1.4.11, Phosphoinositide phospholipase C-gamma-1, Phospholipase C gamma 1

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  WB analysis using 84941-1-RR (same clone as 84941-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84941-1-RR (PLCG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84941-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01700-3

  Sandwich ELISA standard curve of MP01700-3, Human PLCG1 Recombinant Matched Antibody Pair - PBS only. 84941-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag27218. 84941-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP01700-3

  The mean PLCG1 concentration was determined to be 6.50 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

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  IHC staining of human liver cancer using 84941-1-RR (same clone as 84941-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84941-1-RR (PLCG1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84941-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 84941-1-RR (same clone as 84941-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 84941-1-RR (PLCG1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84941-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 84941-1-RR (same clone as 84941-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PLCG1 antibody (84941-1-RR, Clone: 242529A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84941-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 84941-1-RR (same clone as 84941-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PLCG1 antibody (84941-1-RR, Clone: 242529A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84941-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84941-1-PBS bindet in WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test PLCG1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PLCG1 fusion protein Ag27218
• Vollständiger Name: phospholipase C, gamma 1
• Berechnetes Molekulargewicht: 149 kDa
• Gene symbol: PLCG1
• Gene ID (NCBI): 5335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphoinositide phospholipase C-gamma-1 (PLCG1) which belongs to the phosphoinositide-specific phospholipase C (PLC) family, is activated by many extracellular stimuli including hormones, neurotransmitters, and growth factors and modulates several cellular and physiological functions necessary for tumorigeneses such as cell survival, migration, invasion, and angiogenesis by generating inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) via hydrolysis of phosphatidylinositol 4,5-biphosphate (PIP2) (PMID: 9242915). Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (PMID: 2472218). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (PMID: 1708307). It has also been shown that PKA-mediated phosphorylation at Ser1248 is inhibitory to PLCγ1 tyrosine phosphorylation and phospholipase activity in CD3-treated Jurkat cells (PMID: 1370476), suggesting that Ser1248 may be an allosteric regulator of PLCγ1 activity.