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PLCG1 Rekombinanter Antikörper

PLCG1 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242529B11

Kat-Nr. : 84941-4-PBS

Synonyme

Phospholipase C II, Phospholipase C gamma 1, Phosphoinositide phospholipase C-gamma-1, EC:3.1.4.11, 242529B11

Filter:
  • Western Blot (WB) analysis of various lysates using PLCG1 Recombinant antibody (84941-4-RR)

    WB analysis using 84941-4-RR (same clone as 84941-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84941-4-RR (PLCG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84941-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84941-4-RR

    Affinity and Kinetic Characterization of 84941-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84941-4-RR against Human PLCG1 were performed. The affinity constant is 3.90 nM.

Geprüfte Anwendungen

Produktinformation

84941-4-PBS bindet in WB, Indirect ELISA PLCG1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PLCG1 fusion protein Ag27218
Vollständiger Name phospholipase C, gamma 1
Berechnetes Molekulargewicht 149 kDa
Beobachtetes Molekulargewicht 149 kDa
Gene symbol PLCG1
Gene ID (NCBI) 5335
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphoinositide phospholipase C-gamma-1 (PLCG1) which belongs to the phosphoinositide-specific phospholipase C (PLC) family, is activated by many extracellular stimuli including hormones, neurotransmitters, and growth factors and modulates several cellular and physiological functions necessary for tumorigeneses such as cell survival, migration, invasion, and angiogenesis by generating inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) via hydrolysis of phosphatidylinositol 4,5-biphosphate (PIP2) (PMID: 9242915). Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (PMID: 2472218). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (PMID: 1708307). It has also been shown that PKA-mediated phosphorylation at Ser1248 is inhibitory to PLCγ1 tyrosine phosphorylation and phospholipase activity in CD3-treated Jurkat cells (PMID: 1370476), suggesting that Ser1248 may be an allosteric regulator of PLCγ1 activity.