PLCG1 Rekombinanter Antikörper

PLCG1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242529B11

Kat-Nr. : 84941-4-RR

Synonyme

Phospholipase C II, Phospholipase C gamma 1, Phosphoinositide phospholipase C-gamma-1, EC:3.1.4.11, 242529B11

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  WB analysis using 84941-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84941-4-RR (PLCG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84941-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84941-4-RR against Human PLCG1 were performed. The affinity constant is 3.90 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C6-Zellen, Jurkat-Zellen, MCF-7-Zellen, Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84941-4-RR bindet in WB, ELISA PLCG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PLCG1 fusion protein Ag27218
• Vollständiger Name: phospholipase C, gamma 1
• Berechnetes Molekulargewicht: 149 kDa
• Beobachtetes Molekulargewicht: 149 kDa
• Gene symbol: PLCG1
• Gene ID (NCBI): 5335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphoinositide phospholipase C-gamma-1 (PLCG1) which belongs to the phosphoinositide-specific phospholipase C (PLC) family, is activated by many extracellular stimuli including hormones, neurotransmitters, and growth factors and modulates several cellular and physiological functions necessary for tumorigeneses such as cell survival, migration, invasion, and angiogenesis by generating inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) via hydrolysis of phosphatidylinositol 4,5-biphosphate (PIP2) (PMID: 9242915). Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (PMID: 2472218). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (PMID: 1708307). It has also been shown that PKA-mediated phosphorylation at Ser1248 is inhibitory to PLCγ1 tyrosine phosphorylation and phospholipase activity in CD3-treated Jurkat cells (PMID: 1370476), suggesting that Ser1248 may be an allosteric regulator of PLCγ1 activity.