PLCG2 Monoklonaler Antikörper

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PLCG2 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F8H5

Kat-Nr. : 67011-1-PBS

Synonyme

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2, 2F8H5, EC:3.1.4.11, Phosphoinositide phospholipase C-gamma-2, Phospholipase C gamma 2

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  WB analysis of Ramos using 67011-1-Ig (same clone as 67011-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

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  WB analysis of Karpas-422 using 67011-1-Ig (same clone as 67011-1-PBS)

  Karpas-422 cells were subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit spleen using 67011-1-Ig (same clone as 67011-1-PBS)

  Rabbit spleen tissue was subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67011-1-Ig (same clone as 67011-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67011-1-Ig (same clone as 67011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67011-1-Ig (PLCG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67011-1-Ig (same clone as 67011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67011-1-Ig (PLCG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67011-1-PBS bindet in WB, IHC, ELISA PLCG2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PLCG2 fusion protein Ag24355
• Vollständiger Name: phospholipase C, gamma 2 (phosphatidylinositol-specific)
• Berechnetes Molekulargewicht: 148 kDa
• Beobachtetes Molekulargewicht: 148 kDa
• GenBank-Zugangsnummer: BC007565
• Gene symbol: PLCG2
• Gene ID (NCBI): 5336
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phospholipase C-gamma2 (PLCG2) is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor.IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.