PLD1 Polyklonaler Antikörper

PLD1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18355-1-PBS

Synonyme

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  WB analysis using 18355-1-AP (same clone as 18355-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18355-1-AP (PLD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18355-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 18355-1-AP (same clone as 18355-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18355-1-AP (PLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18355-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18355-1-AP (same clone as 18355-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PLD1 antibody (18355-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18355-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18355-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PLD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLD1 fusion protein Ag13227
• Vollständiger Name: phospholipase D1, phosphatidylcholine-specific
• Berechnetes Molekulargewicht: 1074 aa, 124 kDa
• Beobachtetes Molekulargewicht: 112-124 kDa
• GenBank-Zugangsnummer: BC068976
• Gene symbol: PLD1
• Gene ID (NCBI): 5337
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLD1(Phospholipase D1) belongs to the phospholipase D family. It is implicated in the release process of a variety of hormones, most likely at a late stage of exocytosis through the production of lipids that are essential for the fusion process and seem to be also essential for fast neurotransmitter release. PLD1 is primarily localized in vesicular structures such as endosomes, lysosomes, and autophagosomes.