PLD3 Polyklonaler Antikörper

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• KD/KO Validated

PLD3 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 17327-1-AP

Synonyme

Choline phosphatase 3, HindIII K4L homolog, HU K4, Phospholipase D3, PLD 3, PLD3

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Galerie der Validierungsdaten

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  WB analysis using 17327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse brain using 17327-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 17327-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 17327-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF: Maushirngewebe, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• FC: See 1 publications below

Produktinformation

17327-1-AP bindet in WB, IF, FC, ELISA PLD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLD3 fusion protein Ag11306
• Vollständiger Name: phospholipase D family, member 3
• Berechnetes Molekulargewicht: 490 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC036327
• Gene symbol: PLD3
• Gene ID (NCBI): 23646
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Hum Mol Genet; Pathological characterization of a novel mouse model expressing the PD-linked CHCHD2-T61I mutation.; Authors - Teresa R Kee
human	FC	Cell Death Discov; A signature of enhanced lipid metabolism, lipid peroxidation and aldehyde stress in therapy-induced senescence.; Authors - Amy C Flor