PLD3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PLD3 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 17327-1-AP

Synonyme

5'-3' exonuclease PLD3, Choline phosphatase 3, EC:3.1.16.1, HindIII K4L homolog, HU K4

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  WB analysis using 17327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 17327-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 17327-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17327-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17327-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 17327-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 17327-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 17327-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, L02-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

17327-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA PLD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLD3 fusion protein Ag11306
• Vollständiger Name: phospholipase D family, member 3
• Berechnetes Molekulargewicht: 490 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC036327
• Gene symbol: PLD3
• Gene ID (NCBI): 23646
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Hum Mol Genet; Pathological characterization of a novel mouse model expressing the PD-linked CHCHD2-T61I mutation.; Authors - Teresa R Kee
human		Cell Death Discov; A signature of enhanced lipid metabolism, lipid peroxidation and aldehyde stress in therapy-induced senescence.; Authors - Amy C Flor
mouse	WB,IF	ACS Appl Mater Interfaces; FRET Nanosensor Based on DNA Tetrahedron for Visualizing PLD3 Fluctuation in Mouse Models of Alzheimer's Disease; Authors - Shuyan Wen