PLDN Polyklonaler Antikörper

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• KD/KO Validated

PLDN Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(20)

Kat-Nr. : 10891-2-AP

Synonyme

BLOC1S6, PA, PALLID, Pallid protein homolog, Pallidin, pallidin homolog (mouse), PLDN

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  WB analysis using 10891-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10891-2-AP (PLDN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10891-2-AP

  WB result of PLDN antibody (10891-2-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLDN transfected A431 cells.

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  IP experiment of K-562 using 10891-2-AP

  IP result of anti-PLDN (IP:10891-2-AP, 3ug; Detection:10891-2-AP 1:500) with K-562 cells lysate 3600ug.

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  IHC staining of human breast cancer using 10891-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10891-2-AP (PLDN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10891-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10891-2-AP (PLDN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 19 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

10891-2-AP bindet in WB, IP, IF, IHC, ELISA PLDN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLDN fusion protein Ag1337
• Vollständiger Name: pallidin homolog (mouse)
• Berechnetes Molekulargewicht: 23~24 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC004819
• Gene symbol: PLDN
• Gene ID (NCBI): 26258
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pallidin, also named as PLDN and PA, is involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules. It may play a role in intracellular vesicle trafficking, particularly in the vesicle-docking and fusion process. Pallidin interacts with syntaxin-12 which is a t-SNARE protein that mediates vesicle docking and fusion. Pallidin is a 25 kDa subunit of the octamer BLOC-1.

Publikationen

Species	Application	Title
human	WB	Dev Cell; BORC, a Multisubunit Complex that Regulates Lysosome Positioning.; Authors - Jing Pu
human	WB,IF	Curr Biol; BLOC-1 Brings Together the Actin and Microtubule Cytoskeletons to Generate Recycling Endosomes.; Authors - Cédric Delevoye ; KD Validated
human	WB	J Cell Biol; PI4P and BLOC-1 remodel endosomal membranes into tubules; Authors - Riddhi Atul Jani
mouse	WB	Am J Respir Cell Mol Biol; Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.; Authors - Seunghyi Kook ; KO Validated
mouse,human	WB	Elife; BLOS1 mediates kinesin switch during endosomal recycling of LDL receptor.; Authors - Chang Zhang
mouse	WB	Elife; Brucella activates the host RIDD pathway to subvert BLOS1-directed immune defense.; Authors - Kelsey Michelle Wells